Article Last updated: 15-12-2022 Reading time: 7 minutes

AUTOMATED SAMPLE HANDLING ALLOWS A HIGH THROUGHPUT AND

PRECISE SAMPLE DELIVERY

Sample automation for biosensor

Biosensor technology is widely used in biopharmaceutical research as well as in

biomedical diagnosis. Most biosensors make use of a chip or flow cell for detection in

combination with microfluidics. However, sample introduction is still a challenge and is

often executed manually, with all its shortcomings. Therefore, certain suppliers of

permits the maximum

possible efficiency resulting in

the highest sample

significant savings on cost

Biosensor technology

In a biosensor, the bioreceptor (enzyme/antibody/nucleic acid) is designed to interact

with the specific analyte of interest with high selectivity. The general aim of a biosensor is

to enable quick and convenient testing in a wide range of applications, such as disease

Autosamplers are widely used for High Performance Liquid Chromatography (HPLC), as

they are designed to inject precise and accurate sample volumes. However, when

equipped with a modified flow path configuration, they are also ideally suited for

alternative sample separation or detection technology, including automating analytical

processes for biopharmaceutical purposes. So an automated sample preparation

system can be the next, low-level, step in further automating the workflow of laboratory

Alternative flow path configurations

Most of the biosensors are operated at pressures up to 100 psi where sample integrity is

Automated sample preparation system

As described above, most biosensors operate at low pressures. In those cases, the

autosampler can be used to aspirate the sample from the vial into the buffer tubing.

After switching the injection valve, the syringe will dispense the sample towards the

biosensor. This will result in an accurate sample volume dispensed towards the

biosensor. For some applications a constant flow of analyte is required, for example, to

determine the association or dissociation rate with SPR. In those situations the

autosampler syringe can be used to deliver the analyte flow towards the biosensor.

Besides sample injection, the autosampler can also be used to dilute analytes by a fixed

shortcomings of manual injections or complex system configurations. By minimizing

human intervention, margins of error are reduced. Repeatability is guaranteed because

each analyte is treated in exactly the same way. Automated sample dilution or reagent

addition will bring the human intervention for sample handling to a minimum. The

software makes it possible to apply different methods and set up a different method for

each sample, automatically. Because of this efficient and reliable operation, the custom

automated sample preparation system saves a lot on cost per sample and ensures

data consistency.

Integrating and implementing automated

handling with a minimum of instructions. This leaves no reason not to equip your

biosensor with an autosampler that injects the analytes with great precision and

integrity. The implementation of an automated sample preparation system for

biosensor-based research permits the maximum possible efficiency resulting in the

highest sample throughput, leading to significant savings on cost per sample.

Special advantages of Spark’s sample automation technology: